Killam Seminar Series: Microfluidic tools for studying cell-cell interactions
Gr芒ce 脿 la g茅n茅rosit茅 des fiducies Killam, Le Neuro convoque lors d鈥檜ne s茅rie de s茅minaires des conf茅renciers d鈥檈xception dont les travaux passionnent ses chercheurs et ceux de l鈥橴niversit茅 捆绑SM社区.听
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Iain Clark
Professeur adjoint, bio-ing茅nierie, UC Berkeley, 脡tats-Unis
贬么迟别:听jo.stratton [at] mcgill.ca (Jo Anne Stratton)
Abstract:听Central nervous system (CNS)-resident astrocytes and microglia interact to regulate pro-inflammatory programs that drive neurodegenerative diseases, but the pathways and molecules involved are largely unknown. Understanding the language of inflammation is a central question in the field of Neuroimmunology and may guide new therapeutic approaches for multiple sclerosis (MS) and other neurologic diseases. Interactions between CNS-resident cells are highly heterogeneous; astrocytes and microglia nourish and protect neurons, while inflammatory subsets drive demyelination and neurodegeneration in neurologic diseases. We recently developed two novel approaches to study astrocyte-microglia interactions at the single-cell level during inflammation: 1) an in vivo barcoding-based strategy that analyzes the transcriptomes and connections between individual cells, and 2) a droplet-based platform for genome-wide, unbiased CRISPR/Cas9 screening of genes that mediate cell crosstalk. In this seminar, I will discuss the development of these technologies and their application to study astrocyte-microglia signaling in experimental autoimmune encephalomyelitis (EAE), a preclinical mouse model of MS.
